nonlinear dynamic image analysis software  (TotalLab Ltd)

Journal:

Article Title: PKCε overexpression, irrespective of genetic background, sensitizes skin to ultraviolet radiation-induced development of squamous cell carcinomas

doi: 10.1038/jid.2009.212

Figure Lengend Snippet: Heterozygous (+/−) FVB/N K14-T7PKCε mice were crossed for four generations to wildtype (−/−) outcrossed SKH-1 hairless mice for transmission of the transgene and hairlessness. a: Phenotype of female SKH-1K14-T7PKCε transgenic mice. Shown are the photographs of adult SKH-1 hairless PKCε transgenic mouse and its wildtype littermate at 11 weeks of age. b: Skin histology. The dorsal skin from a wildtype and PKCε SKH-1 hairless transgenic mouse was fixed in 10% neutral buffered formalin and embedded in paraffin, and sections (4μm) were stained with hematoxylin and eosin. c: Immunohistochemical staining for PKCε expression, scale bars = 50 μm. d: Epidermal PKC isoform expression profiles in PKCε SKH-1 hairless transgenic mice and their wildtype littermates; results representative of three replicates. e: The quantification of proteins (normalized to β-actin) was done by densitometry analysis using Total Lab Nonlinear Dynamics Image Analysis Software (Nonlinear, USA).

Article Snippet: The dorsal skin from a wildtype and PKCε SKH-1 hairless transgenic mouse was fixed in 10% neutral buffered formalin and embedded in paraffin, and sections (4μm) were stained with hematoxylin and eosin. c : Immunohistochemical staining for PKCε expression, scale bars = 50 μm. d : Epidermal PKC isoform expression profiles in PKCε SKH-1 hairless transgenic mice and their wildtype littermates; results representative of three replicates. e: The quantification of proteins (normalized to β-actin) was done by densitometry analysis using Total Lab Nonlinear Dynamics Image Analysis Software (Nonlinear, USA).

Techniques: Transmission Assay, Transgenic Assay, Staining, Immunohistochemical staining, Expressing, Software

Journal:

Article Title: PKCε overexpression, irrespective of genetic background, sensitizes skin to ultraviolet radiation-induced development of squamous cell carcinomas

doi: 10.1038/jid.2009.212

Figure Lengend Snippet: Female PKCε transgenic SKH-1 hairless mice (TG) and their wildtype littermates (WT) (3 mice per group) were exposed to a single UVR dose (1kJ/m2). The mice were sacrificed at 1, 3, and 6 hrs post UVR exposure. The mouse epidermis was scraped off and total lysate was prepared. Total epidermal lysate from three mice was pooled for the western blot analysis. a and b: The epidermal lysate (25μg protein) was subjected to SDS-PAGE followed by immunoblot analysis using indicated antibodies. Equal loading was confirmed by stripping and reprobing the blots with β-actin antibody. c and d: The quantification of proteins (normalized to β-actin) was done by densitometry analysis using Total Lab Nonlinear Dynamics Image Analysis Software (Nonlinear, USA).

Article Snippet: The dorsal skin from a wildtype and PKCε SKH-1 hairless transgenic mouse was fixed in 10% neutral buffered formalin and embedded in paraffin, and sections (4μm) were stained with hematoxylin and eosin. c : Immunohistochemical staining for PKCε expression, scale bars = 50 μm. d : Epidermal PKC isoform expression profiles in PKCε SKH-1 hairless transgenic mice and their wildtype littermates; results representative of three replicates. e: The quantification of proteins (normalized to β-actin) was done by densitometry analysis using Total Lab Nonlinear Dynamics Image Analysis Software (Nonlinear, USA).

Techniques: Transgenic Assay, Western Blot, SDS Page, Stripping Membranes, Software